Singclean Interleukin-6 (IL-6) Test Kit (Fluorescence Immunochromatography) to help detect inflammation
Interleukin-6 is mainly produced by Th2 cells and secreted by T lymphocytes, B lymphocytes, fibroblasts, endothelial cells, keratinocytes, hepatocytes and bone marrow cells. Its relative molecular weight is 26kDa. It is a glycoprotein composed of 212 amino acid residues. Interleukin-6 is the first biomarker to appear after inflammatory reaction. In different inflammatory diseases, the content of interleukin-6 is significantly different. The increased level of interleukin-6 caused by bacterial infection is significantly higher than that caused by non-bacterial infection, and the level of interleukin-6 is directly proportional to the severity of inflammation and infection. Interleukin-6 is also a sensitive early warning index of sepsis, and has a good correlation with the severity and prognosis of sepsis. As an inflammatory factor, the level of interleukin-6 is also closely related to cardiovascular and cerebrovascular diseases such as atherosclerosis and acute myocardial infarction (AMI).
Indications for Use
The kit is used for quantitative determination of interleukin-6 (IL-6) in human serum, plasma or whole blood samples in vitro.
Who Should Have the Interleukin-6 (IL-6) Test?
Patients suffering from inflammatory conditions;Patients with diabetes, stroke or with cardiovascular disease and C-19-infected patients. Interleukin-6 (IL-6) may be used to help evaluate a person who has a condition associated with inflammation, such as lupus or rheumatoid arthritis, or with infection, such as sepsis. It may also be used in the evaluation of diabetes, stroke, or cardiovascular disease.
Besides, Interleukin-6 (IL-6) Test Kit can help determine the risk of developing severe coagulation-related consequences such as disseminated intravascular coagulation (DIC) and acute respiratory distress syndrome (ARDS) in severe cases of C-19 infection.
Combined with the highly specific PCT joint inspection, it can early warning of sepsis, reflect the severity of infection, evaluate the treatment effect, and help the clinic to quickly judge the patient’s situation
Accurate: the fluorescence immunochromatography method based test kit has higher accuracy.
Easier to read: based on fluorescence immunochromatography, easier to read test result than colloidal gold immunochromatography.
Easy for storage: it can be stored at room temperature (4ºC~30ºC).
Longer validity period: 18 months of validity period.
Product Performance Index
a) Accuracy:When tested with standard IL-6, the relative deviation (B%) between the detection mean value and the target value shall be within ± 15%.
b) Linear range: The linear range is 7pg / ml ~ 5000pg / ml, and the linear correlation coefficient r≥0.9900.
c) Blank limit: not higher than 7ng/mL;
In-batch precision: The coefficient of variation (CV) is not more than 15%;
Precision between batches: The relative range between batches is not more than 15%.
|Interleukin-6 (IL-6) Test Kit (Fluorescence Immunochromatography)
|Whole blood, Plasma, Serum
|10 tests/box, 20 tests/box, 25 tests/box, 30 tests/box, 50 tests/box, 100 tests/box.
|Each pouch contains a test card and a desiccant; the test card is composed of a shell and a test strip, the test strip is composed of sample pad, fluorescent pad (fixed with fluorescent labeled IL-6 monoclonal antibody 1), nitrocellulose membrane (coated with IL-6 monoclonal antibody 2 and goat anti-mouse IgG), filter paper and PVC plastic plate
Fluorescence immunoassay analyzer
It should be purchased separately and can be used for different Test Items, like CK-MB, IL6, PCT.
For different tests, scan the QR code accordingly.
Please read the instructions of the test kit and the manual of the fluorescence immunoanalyzer carefully before use.
a) Bring the test kit and sample to be tested to room temperature.
b) Make sure the ID card matches the batch number of the kit, and insert the ID card into the card reading area of the instrument to read the information.
c) Open the inner package of the test card, take out the test card; draw 75μL of serum plasma sample, drop vertically to the test card sampling place, and start timing; draw 75μL whole blood, vertically drop it to the test card sampling place, and immediately add 1 drop whole blood diluent at the sampling place and start timing.
d) After adding the sample, click “Start Test” on the screen of the fluorescence immunoanalyzer, and the test card will react at room temperature for 15 minutes; insert the test card into the test card slot of the fluorescence immunoanalyzer, and the instrument will automatically test the test card; The test results can be seen on the display screen of the immunoassay analyzer. Click “Print” on the screen to print the results.
Positive Judgment Value or Reference Interval
Normal reference value:<7pg/mL.
Due to differences in geography, race, gender and age, it is recommended that each laboratory establish its own positive judgment value or reference interval.
(For reference only, not used as clinical diagnostic criteria, test results need to be combined with other clinical and laboratory data for clinical diagnosis)
|Content of IL-6(pg/mL)
|Clinical application suggestions
|Indicates mild inflammation or mild infection.
|Suggests general bacterial infection or systemic inflammatory reaction.
|Suggests may be sepsis.
a) This test kit is only for the test of human serum, plasma or whole blood samples. Abnormal hematocrit samples have an impact on the results of the whole blood test. The test results of serum, plasma and whole blood are not significant difference when the hematocrit is between 21% and 48%.
b) The test results need to be combined with other clinical and laboratory data, and if the IL-6 test results do not match the clinical assessment, further testing is required.
c) False positive results can be caused by: cross-reaction of antibody-like components in the blood; some non-specific components in blood have similar antigenic determinants to capture fluorescent-labeled antibodies, such as anisotropic antibodies and rheumatoid factors. In view of the above reasons, the test results should be combined with the patient’s medical history and other laboratory results performed.
d) Hemoglobin, triglycerides and bilirubin in the blood sample can interfere with the test results, where the maximum allowable concentrations are 5g/L, 10g/L and 0.2g/L, respectively.
e) False positive results can be caused by: some unknown components shield the antigenic determinants from binding to the antibody; the unstable IL-6 antigen gradually degrades with time and temperature and cannot be recognized by the antibody. Valid test results depend on a good reagent and sample storage environment.
f) Other factors, including technical reasons, operational errors and other sample factors, may also cause errors in test results.
|All Patients with Inflammation
When to Inspect Your CV Joint
Whenever your car makes a noise, or you notice it taking longer to accelerate than normal, it might be time to look into a CV joint. A CV joint is the component that transmits power to the drive shaft at a constant rotational speed. The difference between a constant and constant-velocity joint is that constant-velocity joints do not increase friction. A constant-velocity joint is mainly used in front-wheel drive vehicles.
Common causes of CV joint failure
Whether your car is a brand new car or a vehicle that has been on the road for years, if you’re experiencing a loud clunking noise or constant vibrations, it may be time to inspect your CV joints. CV joints are the components of your vehicle that connect your front wheels to the axle shafts. They are typically made from iron billets. These parts are lubricated with a special high-temperature CV joint grease.
A CV joint can become damaged over time, but it is possible to detect a bad joint before it is too late. There are several warning signs that you should look for. Some of these signs include dirt, grease, and corrosion. Having a CV joint that is worn out can lead to dangerous vibrations that can make driving unsafe.
Symptoms of a bad CV joint include a knocking noise, shuddering, and loss of power. The noise is usually noticed when accelerating or turning. You may also hear a clicking or grinding sound when turning.
Grease leaks from the CV joint are another sign that you have an issue with the joint. You can easily inspect the joint for leaks by turning the steering wheel to one side. You may also want to check your tires for grease.
The CV joint is usually the weakest link in a vehicle’s suspension system. Because of the constant friction it encounters, it can wear down. This is why it is important to keep it lubricated. Without proper lubrication, the joint may fail early.
Cracks or holes in the boot can also allow dirt and water to get into the joint. This can cause corrosion, damage, and grease leaks. A damaged CV joint boot is the most common cause of CV joint failure.
The boot also serves as a protective covering. It is important that the boot does not leak. If it does, the joint will become damaged and the grease will no longer lubricate it. During a routine vehicle check, you should also inspect the boot for cracks or holes.
Symptoms of a faulty inner CV joint
Symptoms of a faulty inner CV joint include clunking when you decelerate. A clunking sound may also be heard when changing gears on your vehicle. You can test for this by shifting into reverse.
Another less obvious symptom of a faulty CV joint is vibration. Vibrations can be quite a nuisance. They can interfere with your driving ability and safety. These vibrations are especially noticeable at higher speeds and can make driving dangerous.
If you are not sure what a CV joint is, it is a part of your car’s axle that transfers power from the drive shaft to the transmission. This joint is found in front wheel drive, rear wheel drive, and all wheel drive vehicles.
A faulty CV joint can be dangerous, especially if it is not detected early. You could end up with an axle that will no longer allow you to steer your car. A faulty CV joint can also cause shuddering in your axles. It can also lead to the build up of grease on your tires and steering wheel.
The faulty CV joint may also be associated with a clogged transmission fluid filter. This can lead to overheating and transmission slippage. Depending on your vehicle, you may be able to solve the transmission slippage problem by lubricating your CV joints.
The best way to determine if your CV joint is faulty is to do a visual inspection. You can get a closer look at your vehicle’s CV joints by jacking it up. You can also use a boot clamp tool to pull the joint outward.
The CV joint is a complex mechanism. To replace it, you need to follow the manufacturer’s specifications. In most cases, the entire axle assembly will need to be replaced. The cost of the replacement will range from $165 to 800. You will also need to replace the CV boot. You should be able to find a replacement CV boot for your vehicle at a dealer or auto parts store.
The most obvious symptom of a faulty CV joint is a clunking noise. Clunking sounds can be heard at about 30 to 40 MPH. You can also test for clunking by shifting into reverse.
Signs of a blown CV Axle
Keeping an eye out for signs of a blown CV Axle will allow you to reduce damage to your vehicle. If you suspect that you have a problem with your axle, get a professional to inspect the suspension components.
A worn CV Axle can cause a vehicle to shimmy or jerk. This can cause a driver to lose control and make turning more difficult.
A worn CV joint can also cause a knocking sound when turning. It may not seem like a big deal at first, but if the noise is persistent, you may need to take your car to a professional.
If you notice a knocking sound when turning your vehicle, the first thing to check for is the CV Axle boot. The boot protects the CV joint and is typically lubricated. If it is worn or damaged, it can allow dirt and grease to build up. This can also cause a leak.
If you notice a crack in the boot, you may have a problem. You can also turn the wheels at different angles and look for grease near the wheel edges. If this is the case, you may need to replace the boot.
If you have a loud knocking sound, the best solution is to take your vehicle to a mechanic. It may be cheaper to replace the boot than the axle itself.
Other symptoms of a blown CV Axle are excess vibration and noise. These will increase with speed, reducing your comfort and safety. It is also important to avoid driving your vehicle until the problem has been fixed.
Another warning sign of a blown CV Axle is the clunking sound your vehicle makes when accelerating. This is the signal of a damaged inner CV joint.
A damaged CV joint can also cause a loud click or pop when turning. If you hear this noise, you should take your vehicle to a mechanic right away. This is a sign that your CV joint is failing and is not safe to drive.
Lastly, a leaking grease may be the sign of a blown CV Axle. Grease leaks are not as obvious as other symptoms, but they can cause damage to steering and suspension components.
Cost to replace a CV joint
Depending on the make and model of your vehicle, the cost to replace a CV joint can vary. Some people will want to replace the entire CV axle, while others will want to only replace the inner or outer CV joint. Regardless of your decision, you should shop around to find the best price.
The cost of replacing a CV joint is usually around $250 to $350. This price does not include labor costs. In most cases, a trained mechanic will be able to repair your joint.
In some cases, you can find cheap aftermarket parts that will save you a few dollars. However, you may be paying for a cheaper part that will wear out quicker. Make sure you check out the warranty on the part you buy. You can also buy remanufactured parts, which are secondhand parts that are refurbished.
If you are looking to replace the CV joint yourself, you may want to rent a ball joint press. You can order CV joint replacement parts online from most major retailers. You can also purchase OEM (original equipment manufacturer) and aftermarket CV joints. CZPT is a popular brand that will cost between $30 and $75.
A CV axle is a key component in your car. These axles transfer power over bumps and sharp angles. They are exposed to road salts, debris, and extreme temperatures. CV joints can make loud noises, which indicate they are not working correctly. If you hear a knocking sound when accelerating, you may have a CV joint problem. You may also hear a knocking sound when decelerating.
Using a jack stand, you can raise your car to get a closer look at the CV joint. You can also check the transaxle mounts, which are connected to the CV joint. If the transaxle mounts are worn, you may need to replace the CV joint.
If you have a performance car, the cost of a CV joint replacement may triple. You may also have to pay for related repairs. If you cannot afford to pay for repairs, you can sell your car as-is. That will cut the cost of your car down to a more affordable price.
editor by CX 2023-11-22